Last data update: 24 January 2024 16:39 CET
Plasmid name: pREP4 (LMBP 2649)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p2649.gb
(View with Genome Compiler) p2649.txt p2649.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Escherichia coli lac repressor gene (lacI) |
| Promoter: | Escherichia coli lac repressor promoter (lacI) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Neomycin (neo; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid p15A origin |
| Copy number: | Low copy number |
| Host range: | Escherichia coli |
| Parental clone: | pACYC184 |
| Further information: | Because of the presence of the lacI gene, multiple copies of pREP4 ensure high levels of lac repressor and tight regulation of protein expression. Due to mutations in the ribosome binding site of the neomycin resistance gene, pREP4 requires only 25 μg/ml of kanamycin for selection. The plasmid is compatible with all plasmids carrying the ColE1 origin of replication. The nucleotide sequence of the vector corresponds with the EMBL Nucleotide Sequence Database accession number A18755.1. |
| EMBL Accession number: | A18755.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 25/05/1993 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: HindIII, SalI and SphI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by F. Hoffmann - La Roche LTD(1). (1) Basel, Switserland |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 M15 |
| Host reference: | Beckwith et al., Mol. Biol. 8 (1964), 427-430 [PMID: 14168696] |
| Cultivation medium: | LB-Lennox + kanamycin (25 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pREP4 (LMBP 2649) is available at BCCM/GeneCorner. This plasmid was deposited by F. Hoffmann - La Roche LTD. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.