Last data update: 24 January 2024 16:39 CET
Plasmid name: pRS406 (LMBP 4056)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner core plasmid |
GeneCorner sequence: |
p4056.gb
(View with Genome Compiler) p4056.txt p4056.pdf |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Escherichia coli lac Z gene (lacZ); fragment, incl. α part |
Promoter: | Escherichia coli lac operon promoter Phage T7 gene 10 promoter (T7g10) Phage T3 promoter |
Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
Terminator: | - |
Selection marker: | Ampicillin (amp) Saccharomyces cerevisiae URA3; auxotrophic |
Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
Host range: | Escherichia coli Saccharomyces cerevisiae; ura3(-), integrative |
Parental clone: | pBluescriptIISK+ |
Further information: | This is a Yeast Integrating Plasmid (YIp), designed for efficient manipulation of DNA in S. cerevisiae, especially for gene disruptions. The plasmid contains a multi-cloning site (MCS) in the LacZ α-fragment for insert detection using α-complementation in E. coli. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number U03446.1. |
EMBL Accession number: | U03446.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 13/03/2000 |
Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglI/EcoRI, EcoRV, HincII and HindIII. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr Philip Hieter(1). (1) Michael Smith Laboratories, University of British Columbia, Vancouver, Canada |
Plasmid reference: | Sikorski et al., Genetics 122 (1989), 19-27 [PMID: 2659436] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pRS406 (LMBP 4056) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr Philip Hieter and was published in Sikorski et al., 1989. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.