Last data update: 24 January 2024 16:39 CET
Plasmid name: pReceiver-M11-Flag-CARD14-sh (LMBP 7869)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human caspase recruitment domain family member 14 cDNA (CARD14, BIMP2, CARMA2, PSORS2, GeneID 79092); short isoform FLAG epitope tag; N-terminal |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Neomycin (neo; G418) Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | pReceiver-M11 |
| Further information: | The plasmid was constructed by cloning the short isoform of human CARD14 into the pReceiver-M11 vector, in frame with the FLAG epitope. Various mutations in CARD14 correlate to the occurence of the psoriasis disease locus PSORS2. This clone represents the (most abundant) spontaneously active short isoform of CARD14, lacking the MAGUK domain. |
| EMBL Accession number: | - |
| Latest sequence update: | 07/02/2019 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Prof. Dr A. Bowcock(1). (1) Icahn School of Medicine, Mount Sinai, New York, USA |
| Plasmid reference: | Jordan et al., Am. J. Hum. Genet. 90 (2012), 796-808 [PMID: 22521419] [DOI: 10.1016/j.ajhg.2012.03.013] |
| Related plasmid reference: | Jordan et al., Am. J. Hum. Genet. 90 (2012), 784-795 [PMID: 22521418] [DOI: 10.1016/j.ajhg.2012.03.012] |
| Restricted distribution: | - BCCM MTA - Acknowledgment of the depositor in any publication resulting from the use of the MATERIAL |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pReceiver-M11-Flag-CARD14-sh (LMBP 7869) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr A. Bowcock and was published in Jordan et al., 2012. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.