Last data update: 24 January 2024 16:39 CET
Plasmid name: pSCGALCaMET15-1 (LMBP 4212)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4212.gb
(View with Genome Compiler) p4212.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Candida albicans bifunctional cysteine synthase/O-acetylhomoserine aminocarboxypropyltransferase cDNA (MET15, GeneID 3645031); allele 1 |
| Promoter: | Saccharomyces cerevisiae hybrid UDP-glucose 4-epimerase/iso-1-cytochrome C promoter (GAL10/CYC1) |
| Ribosome binding site: |
- |
| Terminator: | Saccharomyces cerevisiae 2 micron plasmid (2μ) FLP terminator Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1) |
| Selection marker: | Ampicillin (amp) Saccharomyces cerevisiae URA3; auxotrophic LEU2 defective (leu2-d; auxotrophic) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Saccharomyces cerevisiae 2 micron plasmid origin (2μ); incl. region conferring stability (STB) |
| Host range: | Escherichia coli Saccharomyces cerevisiae; ura3(-) |
| Parental clone: | pSCGAL10-SN |
| Further information: | The plasmid was constructed by inserting the Candida albicans O-acetylhomoserine O-acetylserine sulfhydrylase cDNA, allele 1 (CaMET15-1), provided by SfiI and NotI linkers between the SfiI and NotI sites of pSCGAL10-SN. Expression of the cloned cDNA is controlled by the regulatable GAL10/CYC1 hybrid promoter from Saccharomyces cerevisiae and the iso-1-cytochrome C (CYC1) and 2μ FLP terminators. The hybrid promoter sequence consists of the upstream activation sequence (UAS-G) of the galactokinase gene (GAL10), the GAL10 promoter and the 5' untranslated leader sequence of the cytochrome C1 (CYC1) gene. The defective LEU2 gene (leu2-d) is poorly expressed, because there is only 30 bp of upstream sequence left in this plasmid; the expression is probably controlled by a promoter within the 2μ sequences. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727323.1. The nucleotide sequence of the CaMET15-1 gene corresponds with the Genbank accession number AF188273.1. Other names of the plasmid are pSCGAL10CaMET15-3 and pSCGALMET15-3. |
| EMBL Accession number: | AF188273.1, view at EMBL, GenBank, DDBJ LT727323.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 02/08/2001 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: NcoI and XhoI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Contreras(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Viaene et al., Yeast 16 (2000), 1205-1215 [PMID: 10992284] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSCGALCaMET15-1 (LMBP 4212) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Contreras . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.