Last data update: 24 January 2024 16:39 CET
Plasmid name: pSCMFE6LMFE6H (LMBP 3221)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p3221.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Saccharomyces cerevisiae α-mating factor 1 gene (MFα1); prepro secretion signal sequence (ppMF) Mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) cDNA; heavy chain (E6H) missing the leader signal sequence Mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) cDNA; light chain (E6L) missing the leader signal sequence |
| Promoter: | Saccharomyces cerevisiae hybrid UDP-glucose 4-epimerase/iso-1-cytochrome C promoter (GAL10/CYC1) Saccharomyces cerevisiae galactokinase promoter (GAL1) |
| Ribosome binding site: |
- |
| Terminator: | Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1) Saccharomyces cerevisiae 2 micron plasmid (2μ) FLP terminator |
| Selection marker: | Ampicillin (amp) Saccharomyces cerevisiae URA3; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Saccharomyces cerevisiae 2 micron plasmid origin (2μ); incl. region conferring stability (STB) |
| Host range: | Escherichia coli Saccharomyces cerevisiae; ura3(-) |
| Parental clone: | pEMBLyex4; pSCUDT2MFE6H; pSCGAL1MFE6L |
| Further information: | This plasmid was constructed as follows: 1) The intermediate plasmid pSCGCMFE6L was constructed by ligating 3 fragments : a) the XbaI/EcoRI vector fragment of pEMBLyex4, b) the EcoRI/SalI fragment of pEMBLyex4, containing the Saccharomyces cerevisiae 2μ origin and c) the SalI/XbaI fragment of pSCGAL1MFE6L, containing the prepro secretion signal sequence (ppMF) of the S. cerevisiae α-mating factor 1 gene (MFα1) fused to cDNA encoding the light chain of the mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ), E6L. 2) The EcoRI fragment of pSCUDT2MFE6H, containing the prepro secretion signal sequence (ppMF) of the S. cerevisiae α-mating factor 1 gene (MFα1) fused to cDNA encoding the heavy chain of the mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ), E6H, was then inserted into the EcoRI opened pSCGCMFE6L vector. The plasmid is used for expression of the heavy chain of the mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ): E6H. |
| EMBL Accession number: | - |
| Latest sequence update: | 28/08/2001 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Contreras(1) (2). It was constructed by W. Lammerant(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium. |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSCMFE6LMFE6H (LMBP 3221) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Contreras . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.