Last data update: 24 January 2024 16:39 CET
Plasmid name: pSCTyGAL1mBAX-L (LMBP 3871)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p3871.gb
(View with Genome Compiler) p3871.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse Bcl2-associated X cDNA (Bax, BAX); α variant |
| Promoter: | Saccharomyces cerevisiae galactokinase promoter (GAL1) |
| Ribosome binding site: |
- |
| Terminator: | Saccharomyces cerevisiae 2 micron plasmid (2μ) FLP terminator Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1) |
| Selection marker: | Ampicillin (amp) LEU2; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Saccharomyces cerevisiae; leu2(-), integrative |
| Parental clone: | pSCTyGAL1-L; pUC19muBAX |
| Further information: | The plasmid was constructed by inserting the XbaI-HindIII fragment from pUC19muBAX, containing the α variant of the mouse Bcl2-associated X cDNA (mBAX), into the XbaI-HindIII opened pSCTyGAL1-L plasmid. pSCTyGAL1mBAX-L is an integrative expression plasmid for the mouse Bax-α protein in Saccharomyces cerevisiae. After XhoI linearisation in its Ty1 δ element, multicopy genomical integration leads to stable expression. The nucleotide sequence of the mBAX cDNA corresponds with the EMBL Nucleotide Sequence Database accession number L22472.1. Other names of the plasmid are YIpUTyL-muBAX, YIpUTyLB and pSCTyGALmBAX. |
| EMBL Accession number: | L22472.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 16/06/1998 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglI/SphI, HindIII/XbaI, MluI and PvuII/XmnI. The XmnI site at position 4143 could not be experimentally confirmed. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Contreras(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Reekmans et al., FEMS Yeast Res. 5 (2005), 711-725 [PMID: 15851100] [DOI: 10.1016/j.femsyr.2004.12.010] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSCTyGAL1mBAX-L (LMBP 3871) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Contreras and was published in Reekmans et al., 2005. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.