Last data update: 24 January 2024 16:39 CET
Plasmid name: pSCUDT2AGMFE6F1 (LMBP 3187)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | not available |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Saccharomyces cerevisiae α-mating factor 1 gene (MFα1); prepro secretion signal sequence (ppMF) Mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) cDNA; Fab fragment (E6F1) |
Promoter: | Saccharomyces cerevisiae iso-1-cytochrome C promoter (CYC1) Chicken β-actin/rabbit β-globin hybrid promoter (AG) |
Ribosome binding site: |
- |
Terminator: | Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1) |
Selection marker: | Ampicillin (amp) Saccharomyces cerevisiae URA3; auxotrophic Methotrexate (MTX) |
Replicon: | Escherichia coli plasmid pMB1 origin Saccharomyces cerevisiae 2 micron plasmid origin (2μ) |
Host range: | Escherichia coli Saccharomyces cerevisiae; ura3(-) |
Parental clone: | pUDT2 |
Further information: | The plasmid is used for the expression of the Fab fragment (E6F1) of the mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ). |
EMBL Accession number: | - |
Latest sequence update: | 02/06/2003 |
Authenticity test: | By lack of sequence data, it was not possible to perform the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Dr K. De Sutter(1) (2) and Prof. Dr R. Contreras(1) (2). It was constructed by W. Lammerant(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium. |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061 |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSCUDT2AGMFE6F1 (LMBP 3187) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. De Sutter and Prof. Dr R. Contreras . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.