Last data update: 24 January 2024 16:39 CET
Plasmid name: pSEVA321 (LMBP 12704)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p12704.gb |
| Sequence analysis results Genecorner: |
NGS: f12-gc-may2021.fasta |
| Cloned DNA: |
Broad-host-range plasmid RK2 trans-acting replication initiator protein DNA (trfA) |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator Phage λ T0 terminator |
| Selection marker: | Chloramphenicol (cam) |
| Replicon: | Broad-host-range plasmid RK2/RP4 origin of transfer (oriT) |
| Host range: | Escherichia coli Gram-negative bacterial strains |
| Further information: | The plasmid was constructed by combining its synthetic modules. The nucleotide sequence of the pSEVA321 vector corresponds with the EMBL Nucleotide Sequence Database accession number JX560332.1. |
| EMBL Accession number: | JX560332.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 29/03/2021 |
| Authenticity test: | This plasmid has been fully sequenced but the NGS sequence data still need to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr E. Martinez(1) and Prof. Dr V. De Lorenzo(1). (1) Environmental Synthetic Biology Laboratory, National Center for Biotechnology, Madrid, Spain |
| Plasmid reference: | Silva-Rocha et al., Nucleic Acids Res. 41 (2013), D666-675 [PMID: 23180763] [DOI: 10.1093/nar/gks1119] |
| Related plasmid reference: | Martinez-Garcia et al., Nucleic Acids Res. 48 (2020), D1164-1170 [PMID: 31740968] [DOI: 10.1093/nar/gkz1024] Martinez-Garcia, Nucleic Acids Res. 43 (2015), D1183-1189 [PMID: 25392407] [DOI: 10.1093/nar/gku1114] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 CC118 |
| Host reference: | Manoil and Beckwith, Proc. Natl. Acad. Sci. U.S.A. 82 (1985), 8129-33 [PMID: 2999794] [DOI: 10.1073/pnas.82.23.8129] |
| Related host reference: | Herrero et al., J. Bacteriol. 172 (1990), 6557-6567 [PMID: 2172216] [DOI: 10.1128/jb.172.11.6557-6567.1990] Arif et al., Bangladesh J. Microbiol. 31 (2014), 1-8 [DOI: 10.3329/bjm.v31i1.28457] |
| Cultivation medium: | LB-Lennox + chloramphenicol (34 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSEVA321 (LMBP 12704) is available at BCCM/GeneCorner. This plasmid was deposited by Dr E. Martinez and Prof. Dr V. De Lorenzo and was published in Silva-Rocha et al., 2013. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.