Last data update: 24 January 2024 16:39 CET
Plasmid name: pSGCG (LMBP 9561)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p9561.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Bacillus subtilis levansucrase cDNA (sacB, GeneID 936413) Escherichia coli insertion sequence IS1 insB Discosoma sp. red fluorescent (tetrameric) protein cDNA (DsRed); mutant (mCherry) Aequorea victoria green fluorescent protein DNA (GFP); mutated fragment |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Streptomycin (Sm) |
| Replicon: | Escherichia coli plasmid R6K origin; defective pir gene Broad-host-range plasmid RK2/RP4 origin of transfer (oriT) |
| Host range: | Escherichia coli; use strains supplying the R6K pir function Gram-negative bacterial strains |
| Parental clone: | pKNG101 |
| Further information: | The plasmid was constructed by cloning the mCherry fluorescent protein, flanked by the 5' and 3' fragments of mutant GFP, as a BglII fragment into the BamHI opened pKNG101 suicide vector. The plasmid is meant for allelic exchange, to replace the fluorescent GFP marker by mCherry. Introduce the plasmid by electroporation, or through conjugation, using plasmid RK2/RP4 transfer functions (provided in trans by RK2/RP4, RK2/RP4-derived vectors, other replicons carrying RK2/RP4 transfer functions (e.g. pRK2073) or strains expressing the tra genes of RK2/RP4). Take a low-copy-plasmid yield into account while choosing a plasmid DNA isolation procedure. In Gengler et al. (2015) the host/plasmid combination is named Escherichia coli 10WP. |
| EMBL Accession number: | - |
| Latest sequence update: | 23/03/2015 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: KpnI, NsiI/SalI, PstI and SpeI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Dr. P. Wattiau (1). It was constructed by Dr. S. Gengler (2)(3). (1) CODA-CERVA, Department of Bacterial Diseases, Brussels, Belgium (2) Veterinary and Agrochemical Research Centre, Brussels, Belgium (3) Institute of life sciences, Catholic University of Louvain-la-Neuve, Louvain-la-Neuve, Belgium. |
| Plasmid reference: | Gengler et al., PLoS One. 10 (2015), e0116818 [PMID: 25635766] [DOI: 10.1371/journal.pone.0116818] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 SM10(λpir) |
| Host reference: | - |
| Related host reference: | Simon et al., Biotechnology 1 (1983), 784-791 [DOI: 10.1038/nbt1183-784] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) + streptomycin (25 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSGCG (LMBP 9561) is available at BCCM/GeneCorner. The plasmid was deposited by Dr. P. Wattiau and was published in Gengler et al., 2015. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.