Last data update: 24 January 2024 16:39 CET
Plasmid name: pSI4008 (LMBP 9290)
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Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | not available |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Yersinia enterocolitica arsenite pump transmembrane protein gene (arsB) Yersinia enterocolitica arsenate reductase gene (arsC) Yersinia enterocolitica arsenic resistance protein gene (arsH) Yersinia enterocolitica arsenite inducible repressor gene (arsR) Yersinia enterocolitica low calcium response protein genes (lcrD, lcrG, lcrR and lcrV) Yersinia enterocolitica non-specific endonuclease gene (nuc) Yersinia enterocolitica positive transcriptional regulator gene (pprA) Yersinia enterocolitica plasmid pYV replication protein genes (repA and repB) Yersinia enterocolitica plasmid pYV replication protein gene (repB) Yersinia enterocolitica transcriptional regulator gene (spyA) Yersinia enterocolitica DNA binding protein gene (spyB) Yersinia enterocolitica specific yop chaperone gene (sycD, sycE, sycH, sycN and sycT) Yersinia enterocolitica transposase gene (tnpA) Yersinia enterocolitica resolvase gene (tnpR) Yersinia enterocolitica DNA helicase gene (traI) Yersinia enterocolitica IncF plasmid conjugative transfer pilin acetylase gene (traX) Yersinia enterocolitica yop release control protein gene (tyeA) Yersinia enterocolitica transcriptional activator gene (virF) Yersinia enterocolitica lipoprotein gene (virG) Yersinia enterocolitica outer membrane adhesin protein gene (yadA) Yersinia enterocolitica surface exclusion protein gene (ylpA) Yersinia enterocolitica integral membrane protein gene (yomA) Yersinia enterocolitica anti-host protein genes (yopB, yopD, yopE, yopM, yopN, yopO, yopP, yopQ and yopT) Yersinia enterocolitica anti-host protein H gene (yopH); inactive fragment (yopHΔ1-1011) Yersinia enterocolitica secretion protein genes (yscA, yscB, yscC, yscD, yscE, yscF, yscG, yscH, yscI, yscJ, yscK, yscL, yscM1, yscM2, yscN, yscO, yscP, yscQ, yscR, yscS, yscT, yscU, yscV, yscW, yscX and yscY) |
Promoter: | - |
Ribosome binding site: |
- |
Terminator: | - |
Selection marker: | Arsenic (ars) |
Replicon: | Yersinia enterocolitica plasmid pYV40 ori |
Host range: | Yersinia enterocolitica |
Parental clone: | pYV40; pAB31 |
Further information: | The plasmid was constructed via allelic exchange between the Y. enterocolitica yopH gene of the pYV MRS40 and the yopHΔ1-1011 fragment on the suicide plasmid pAB31. The Y. enterocolitica yopHΔ1-1011 gene fragment lacks the first 352 codons, including the start codon, only the last 115 codons are left. |
EMBL Accession number: | - |
Latest sequence update: | 03/07/2014 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr G. Cornelis(1). It was constructed by Dr I. Stainier(2). (1) Research Unit in Microorganism Biology, University of Namur, Namur, Belgium (2) Catholic University of Louvain, Louvain-la-Neuve, Belgium |
Plasmid reference: | Mills et al., Proc. Natl. Acad. Sci. U.S.A. 94 (1997), 12638-12643 [PMID: 9356502] |
Related plasmid reference: | Sory et al., Proc. Natl. Acad. Sci. U.S.A. 92 (1995), 11998-12002 [PMID: 8618831] |
Restricted distribution: | - BCCM MTA |
Distributed as: | active culture or plasmid DNA |
Host for distribution: | Yersinia enterocolitica E40 |
Host reference: | - |
Cultivation medium: | BHI + arsenic (400 μM) + nalidixic acid (35 μg/ml) |
Cultivation temperature: | 20°C |
Biosafety level: | L2 |
Cultivation remark: | Cultivate on LB agar plates, or in liquid BHI. |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSI4008 (LMBP 9290) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Cornelis and was published in Mills et al., 1997. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.