Last data update: 24 January 2024 16:39 CET
Plasmid name: pSOD2 (LMBP 5013)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p5013.gb
(View with Genome Compiler) p5013.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Nicotiana plumbaginifolia iron superoxide dismutase cDNA (FeSOD); fragment Escherichia coli lac Z gene (lacZ); fragment |
| Promoter: | Escherichia coli lac operon promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pUC18 |
| Further information: | The plasmid was constructed as follows: 1) a cDNA expression library from a Nicotiana plumbaginifolia (tobacco) heterotrophic cell suspension culture was constructed in pUC18; 2) a clone synthesizing a SOD enzyme with characteristic properties of a tobacco chloroplastic iron superoxide dismutase cDNA (FeSOD), resistant to KCN and sensitive to H2O2, was selected. The inserted FeSOD fragment is fused to lacZα and expressed under control of the lacZ promoter. The first 25 amino acid codons of FeSOD are missing. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727068.1. The nucleotide sequence of the FeSOD cDNA corresponds with the Genbank accession number M55909.1. |
| EMBL Accession number: | M55909.1, view at EMBL, GenBank, DDBJ LT727068.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 07/06/2005 |
| Sequence detail: | Nucleotide sequence at the 5' side of the FeSOD coding sequence:
| lacZ alpha part | polylinker of pUC18
5'...ATG.ACC.ATG.ATT.ACG.AAT.TCG.AGC.TCG.GTA.CCC.GGG.GAT.CCT.CTA.
EcoRI
|--> FeSOD
| G-tail | 26 30
GAG.TCG.ACC.TGC.AGG.GGG.GGG.GGG.GGG.GCT.AAA.TTT.GAA.CTC.CAG.C...3'
PstI |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI, NcoI, NdeI and PstI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr D. Inzé(1) (2) and constructed by W. Van Camp(1) (2). (1) Department of Plant Systems Biology, VIB, Ghent, Belgium (2) Department of Plant Systems Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Van Camp et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 9903-9907 [PMID: 2263641] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSOD2 (LMBP 5013) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr D. Inzé and constructed by W. Van Camp and was published in Van Camp et al., 1990. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.