Last data update: 24 January 2024 16:39 CET
Plasmid name: pSOS-icTNF (LMBP 5274)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
NGS: a11-gc-dec2018.fasta |
| Cloned DNA: |
Human bait protein for the Ras pathway (Sos) Human tumor necrosis factor (TNF); intracellular part of membrane-bound TNF |
| Promoter: | Saccharomyces cerevisiae alcohol dehydrogenase promoter (ADH1) |
| Ribosome binding site: |
- |
| Terminator: | Saccharomyces cerevisiae alcohol dehydrogenase terminator (ADH1) |
| Selection marker: | Ampicillin (amp) LEU2; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin Saccharomyces cerevisiae 2 micron plasmid origin (2μ) |
| Host range: | Escherichia coli Saccharomyces cerevisiae; leu2(-) |
| Parental clone: | pSos |
| Further information: | The plasmid was constructed by PCR amplifying the intracellular part of human TNF (icTNF) and cloning it into the BamHI/SalI opened pSOS vector. The plasmid is useful for SOS recruitment of yeast two-hybrid systems with the intracellular part of membrane-bound TNF. |
| EMBL Accession number: | - |
| Latest sequence update: | 08/06/2006 |
| Authenticity test: | This plasmid has been fully sequenced but the NGS sequence data still need to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | CytoTrap Vector Kit Instruction Manual (Agilent Technologies) |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
| Related website: | http://www.chem.agilent.com/en-US/Search/Pages/SearchResults.aspx?k=CytoTrap%20manual&a=scope:%22English%22 |
Refer in your Materials and Methods: |
pSOS-icTNF (LMBP 5274) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.