Last data update: 24 January 2024 16:39 CET
Plasmid name: pSP64GNNT (LMBP 1749)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p1749.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
- |
Promoter: | Phage SP6 promoter Escherichia coli lac operon promoter |
Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
Terminator: | Human interferon α 14 gene terminator (IFNA) |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli |
Parental clone: | pSP64GNN |
Further information: | The terminator sequence of human interferon α 14 was isolated as an AvaI-HindIII fragment from pSP65IFNAT, from which the HindIII site was filled in (Klenow DNA polymerase) and provided with an EcoRI linker. This fragment was then inserted into the AvaI-EcoRI opened pSP64GNN plasmid. See also pSP64GNN, pSP64T and pSP65IFNAT for basic constructions. E-AvaI, E-BglII, E-NotI, E-PstI and XmaIII are not unique. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. Other name of the plasmid is pSP64TNNT. |
EMBL Accession number: | - |
Latest sequence update: | 22/05/1989 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by L. Stuyver(1) and Prof. Dr R. Contreras(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Related plasmid reference: | Henco et al., J. Mol. Biol. 185 (1985), 227-260 [PMID: 4057246] Krieg et al., Nucleic Acids Res. 12 (1984), 7057-7070 [PMID: 6207484] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061 |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSP64GNNT (LMBP 1749) is available at BCCM/GeneCorner. This plasmid was deposited by L. Stuyver and Prof. Dr R. Contreras. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.