Last data update: 24 January 2024 16:39 CET
Plasmid name: pSP64GcHIL6 (LMBP 1956)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p1956.gb
(View with Genome Compiler) p1956.txt p1956.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human interleukin 6 cDNA (IL6, IL-6, IFNB2, BSF2, GeneID 3569) |
| Promoter: | Escherichia coli lac operon promoter Phage SP6 promoter |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pSP64T; pURgHIL61; pBRcHIL6f3 |
| Further information: | The construction of this vector is described in the publication of Poupart et al. (1987). This plasmid contains the entire hIL6 coding sequence, cloned in the synthetic BglII site of pSP64T between the 5' UTR and 3' UTR regions of the Xenopus laevis β-globin gene, under control of the SP6 promoter. It is possible that the sequence information is incorrect at position 51, and hence that there is a BalI site preceding the BglII site. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727607.1. The nucleotide sequence of the hIL6 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number M18403.1. Name mentioned in Poupart et al. (1987) is pSP64T-26K. |
| EMBL Accession number: | M18403.1, view at EMBL, GenBank, DDBJ LT727607.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 24/10/2003 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: AccI, BglII, StyI and TaqI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr G. Haegeman(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Poupart et al., EMBO J. 6 (1987), 1219-1224 [PMID: 3497029] |
| Related plasmid reference: | Krieg et al., Nucleic Acids Res. 12 (1984), 7057-7070 [PMID: 6207484] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061(B) |
| Host reference: | - |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSP64GcHIL6 (LMBP 1956) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Haegeman and was published in Poupart et al., 1987. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.