Last data update: 24 January 2024 16:39 CET
Plasmid name: pSP65HIL4f (LMBP 1918)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p1918.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human interleukin 4 cDNA (IL4, IL-4, BCGF-1, BCGF1, BSF1, GeneID 3565); fragment |
| Promoter: | Phage SP6 promoter Escherichia coli lac operon promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pSP65 |
| Further information: | The plasmid was constructed by inserting the first EcoRI fragment of the EcoRI linked hIL4 cDNA gene into the unique EcoRI site of pSP65, in the antisense orientation relative to the SP6 promoter. The first EcoRI linker was placed approx. 60 nucleotides upstream from the start codon of the hIL4 cDNA; the natural EcoRI site is located 100 nucleotides upstream from the termination codon of the hIL4 cDNA. This plasmid is useful for preparing antisense RNA probes. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. The hIL4 cDNA was not sequenced but assumed to be identical to the sequence reported by Yokota et al. (1986). |
| EMBL Accession number: | - |
| Latest sequence update: | 13/02/1989 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr W. Fiers(1) (2). It was constructed by B. Jayaram(1) (2) and R. Devos(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Yokota et al., Proc. Natl. Acad. Sci. U.S.A. 83 (1986), 5894-5898 [PMID: 3016727] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSP65HIL4f (LMBP 1918) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr W. Fiers . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.