Last data update: 24 January 2024 16:39 CET
Plasmid name: pSP72-E-OPTN (LMBP 5937)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p5937.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human optineurin cDNA (OPTN, FIP2, GLC1E, HYPL, HIP7, GeneID 10133) E-tag; N-terminal |
| Promoter: | Phage SP6 promoter Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pSP72; pCAGGS-E-hOPTN |
| Further information: | The plasmid was constructed by inserting the EcoRI-XhoI fragment of pCAGGS-E-hOPTN, containing the human OPTN coding sequence fused to the N-terminal E-tag, into the EcoRI-XhoI opened pSP72 vector. The nucleotide sequence of pSP72 corresponds with the EMBL Nucleotide Sequence Database accession number X65332.2. The nucleotide sequence of the human OPTN cDNA corresponds with the EMBL Nucleotide Sequence Database accession number BC013876.2 but contains a silent mutation at amino acid position 34. |
| EMBL Accession number: | X65332.2, view at EMBL, GenBank, DDBJ BC013876.2, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 04/02/2009 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSP72-E-OPTN (LMBP 5937) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.