Last data update: 24 January 2024 16:39 CET
Plasmid name: pSP72-E-hA20 (LMBP 5931)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p5931.gb
(View with Genome Compiler) p5931.txt p5931.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human TNF alpha induced protein 3 cDNA (TNFAIP3, A20, OTUD7C, GeneID 7128) E-tag; N-terminal |
| Promoter: | Phage SP6 promoter Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pSP72; pCAGGSEhA20 |
| Further information: | The E-hA20 sequence was cut out of the pCAGGSEhA20 vector with EcoRI and XhoI and cloned into the EcoRI-XhoI opened pSP72 vector. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726816.1. The nucleotide sequence of pSP72 corresponds with the EMBL Nucleotide Sequence Database accession number X65332.2. The nucleotide sequence of the hA20 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number M59465.1. |
| EMBL Accession number: | X65332.2, view at EMBL, GenBank, DDBJ M59465.1, view at EMBL, GenBank, DDBJ LT726816.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 20/02/2009 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: Alw44I, Bgl/ScaI, EcoRV/SacI, PvuI, SphI/SspI and XmnI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). It was constructed by Jens Staal(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Krieg et al., Methods Enzymol. 155 (1987), 397-415 [PMID: 2828872] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSP72-E-hA20 (LMBP 5931) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.