Last data update: 24 January 2024 16:39 CET
Plasmid name: pSP72-E-hA20-R439A (LMBP 5975)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human TNF alpha induced protein 3 cDNA (TNFAIP3, A20, OTUD7C, GeneID 7128); mutated sequence E-tag; N-terminal |
| Promoter: | Phage SP6 promoter Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pSP72; pCAGGS-E-A20-R439A |
| Further information: | The hA20 mutated sequence was amplified by PCR and inserted as a EcoRI-XhoI fragment into the EcoRI-XhoI opened pSP72 vector. The hA20 coding sequence contains an R439A mutation. The nucleotide sequence of pSP72 corresponds with the EMBL Nucleotide Sequence Database accession number X65332.2. The nucleotide sequence of the wt hA20 cDNA was obtained from the EMBL Nucleotide Sequence Database accession number M59465.1. |
| EMBL Accession number: | X65332.2, view at EMBL, GenBank, DDBJ M59465.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 09/09/2008 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Jens Staal(1) (2) and Prof. Dr R. Beyaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Krieg et al., Methods Enzymol. 155 (1987), 397-415 [PMID: 2828872] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSP72-E-hA20-R439A (LMBP 5975) is available at BCCM/GeneCorner. This plasmid was deposited by Jens Staal and Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.