Last data update: 24 January 2024 16:39 CET
Plasmid name: pSSU101 (LMBP 1732)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Suppressor tRNA gene (supF) |
| Promoter: | Suppressor tRNA gene promoter (supF) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid ColE1 origin |
| Host range: | Escherichia coli |
| Parental clone: | - |
| Further information: | The synthetic suppressor tRNA gene (SupF) carries an EcoRI site at both termini. The length of this synthetic gene is 209 nucleotides (including the EcoRI sites). The synthetic suppressor gene was phosphorylated at the 5' ends and then joined to an ColE1-amp(R) vector after the latter had been digested by EcoRI. Expression of the supF gene is controlled by its own promoter. The available information is insufficient to draw a map. |
| EMBL Accession number: | - |
| Latest sequence update: | 28/03/1990 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr D. Söll(1). (1) Yale University, Connecticut, USA |
| Plasmid reference: | - |
| Related plasmid reference: | So et al., Mol. Gen. Genet. 142 (1975), 239-249 [PMID: 1045010] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSSU101 (LMBP 1732) is available at BCCM/GeneCorner. This plasmid was deposited by Dr D. Söll. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.