Last data update: 24 January 2024 16:39 CET
Plasmid name: pSTW14 (LMBP 9314)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p9314.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Yersinia enterocolitica secretion protein P gene (yscP); modified sequence (yscP686) Myc epitope; N-terminal Histidine tag (His-tag); N-terminal |
Promoter: | Escherichia coli L-arabinose operon promoter (araB, araBAD, BAD) |
Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli L-ribulokinase gene (araB); optimized sequence |
Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli |
Parental clone: | pSTW4; pBAD/Myc-HisA |
Further information: | The plasmid was constructed by cloning a fragment of Y. enterocolitica yscP (codons 214-374) into pSTW4, between codons 250 and 251 of Y. enterocolitica yscP. This results in the Y. enterocolitica yscP686 cloned in the pBAD/Myc-HisA vector. Other names of the plasmid are pBAD:yscP686+ and pBAD/Myc-HisA-yscP686. |
EMBL Accession number: | - |
Latest sequence update: | 03/07/2014 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr G. Cornelis(1). It was constructed by Dr S. Wagner(2). (1) Research Unit in Microorganism Biology, University of Namur, Namur, Belgium (2) Biozentrum, University of Basel, Switzerland |
Plasmid reference: | Wagner et al., Proc. Natl. Acad. Sci. U.S.A. 107 (2010), 13860-13865 [PMID: 20643949] |
Restricted distribution: | - BCCM MTA |
Distributed as: | active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 LK111 |
Host reference: | Zabeau et al., EMBO J. 1 (1982), 1217-1224 [PMID: 6327257] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSTW14 (LMBP 9314) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Cornelis and was published in Wagner et al., 2010. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.