Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV/TXBP15-1 (LMBP 3514)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p3514.gb
(View with Genome Compiler) p3514.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human Tax1 binding protein 1 cDNA (TAX1BP1, T6BP, TXBP151, GeneID 8887); small form (TAX1BP1-S) |
| Promoter: | Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSG424 |
| Further information: | This plasmid was constructed by inserting the splice variant of the human Tax1 binding protein 1 cDNA (TAX1BP1-S) as a BamHI-SpeI fragment between the BglII and XbaI sites of pSG424 (Clontech), replacing the GAL4 DNA-binding domain. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727345.1. Referring to the pCAGGS-TAX1BP1-S sequence analysis results of the Department of Biomedical Molecular Biology (Ghent University, Belgium), the human TAX1BP1-S coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number U33821.2, except for the nucleotides CG at position 147-148 being substituted by GC, resulting in H49Q and V50L mutations, and nucleotide T at position 1203 being substituted by C, resulting in a silent mutation. These differences were confirmed by Dr K-T. Jeang (NIH, Bethesda, Maryland, USA). The C-terminal coding region of TAX1BP1-S and the 3' UTR region have been sequenced at the Department of Biomedical Molecular Biology (Ghent University, Belgium), confirming the T-->C nucleotide substitution described above. Other name of the plasmid is pSG424TAXBP151. |
| EMBL Accession number: | U33821.2, view at EMBL, GenBank, DDBJ LT727345.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 12/04/2002 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI/HindIII, BglII/XbaI, EcoRI, HindII, NcoI/SpeI and XmnI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr Kuan-Teh Jeang(1). (1) NIH, Bethesda, Maryland, USA |
| Plasmid reference: | - |
| Related plasmid reference: | Sadowski et al., Nucleic Acids Res. 17 (1989), 7539 [PMID: 2798115] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV/TXBP15-1 (LMBP 3514) is available at BCCM/GeneCorner. This plasmid was deposited by Dr Kuan-Teh Jeang. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.