Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV20S (LMBP 734)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | psv20s.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pAT153; pSV2betaG |
| Further information: | Construction: SV40 part ranging from the EcoRI site clockwise to nucleotide 989; junction between VP1 and polyA signal with the splicing signal of the small t-antigen via the BclI site (SV40 coordinate 2770) and the MboI site (SV40 coordinate 4100); junction between the splicing signal of the small t-antigen and SV40 early promoter: via the MboI site (SV40 coordinate 4710) and a HindIII linker with a HindIII-PvuII fragment containing the promoter; junction between SV40 early promoter and pAT153: via PvuII site and filled- in AvaI site from pAT153 (pAT153 coordinate 1425).(see PhD thesis p130) Contains the SV40 small t-antigen splicing signal. Since prokaryotic part is derived from pAT153, there are no 'poison' sequences present. Designed for transient expression in COS cells and constitutive expression in CHO cells (cotransfection with DHFR plasmid). Expression sites: a unique HindIII site. It is presumably possible to clone into the AvrII and/or StuI sites for expression, but this has not been tested. Other name of the plasmid is pSV2d. |
| EMBL Accession number: | - |
| Latest sequence update: | 16/02/1989 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by D. Huylebroeck(1) and Prof. Dr W. Min Jou(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | PhD thesis Danny Huylebroeck (1985) |
| Related plasmid reference: | Huylebroeck et al., Gene 66 (1988), 163-181 [PMID: 2844629] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli |
| Host reference: | - |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV20S (LMBP 734) is available at BCCM/GeneCorner. This plasmid was deposited by D. Huylebroeck and Prof. Dr W. Min Jou and was published in Danny Huylebroeck, 1985. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.