Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV23IVNAsm1 (LMBP 3759)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p3759.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Influenza A virus (A/Victoria/3/1975(H3N2)) neuraminidase cDNA (NA, IVNA); mutated fragment, N-terminal anchor free Influenza A virus (A/Victoria/3/1975(H3N2)) haemagglutinin cDNA (HA, IVHA); signal sequence |
| Promoter: | Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV23IVNAs |
| Further information: | This plasmid was constructed by site-specific mutagenesis, creating a second StuI restriction site. The A/Victoria/3/75 influenza neuraminidase gene is excisable as a StuI-XbaI fragment starting at amino acid 52. Codon 51 of the neuraminidase gene (ATG, Met) was mutated to AGG (Arg). Due to an error in the primer sequence, codon 57 (Ile) was unintentionally changed to Ser. The nucleotide sequence of wild type IVNA corresponds with the EMBL Nucleotide Sequence Database accession number CY015510.1. Other name of the plasmid is pSV23IVNAs52. |
| EMBL Accession number: | CY015510.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 18/11/1997 |
| Sequence detail: | A/Victoria/3/75 influenza neuraminidase: wild type
50 55
5' GCG AAC AAC CAA GTA ATG CCG TGT GAA CCA ATA ATA ATA GAA 3'
Ala Asn Asn Gln Val Met Pro Cys Glu Pro Ile Ile Ile Glu
Mutated A/Victoria/3/75 influenza neuraminidase:
50 55
5' GCG AAC AAC CAA GTA AGG CCT TGT GAA CCA ATA TCA ATA GAA 3'
Ala Asn Asn Gln Val Arg Pro Cys Glu Pro Ile Ser Ile Glu
* * **
StuI
Primer sequence:
5' GCG AAC AAC CAA GTA AGG CCT TGT GAA CCA ATA TC 3'
StuI
*: mutated nucleotide |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr N. Callewaert(1) (2). It was constructed by S. Geysens(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | PhD thesis Steven Geysens (1997) |
| Related plasmid reference: | Morinaga et al., Biotechnology 2 (1984), 636-639 |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV23IVNAsm1 (LMBP 3759) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr N. Callewaert and was published in Steven Geysens, 1997. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.