Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV23SHTNF (LMBP 2173)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p2173.gb
(View with Genome Compiler) p2173.txt p2173.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human tumor necrosis factor cDNA (TNF) |
| Promoter: | Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV23S; pATHTNF |
| Further information: | This plasmid was constructed as follows: the human TNF cDNA from pATHTNF was inserted into the unique SalI site of pSV23S after SalI linkers (CGTCGACG) were ligated to the PstI sites of the human TNF cDNA (blunted with T4 exonuclease). The length of the dG-tail at the 5' UTR of the human TNF cDNA is not known. The 3' UTR of the human TNF cDNA is not complete. This plasmid contains the small t-antigen splicing signal. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727630.1. The nucleotide sequence of the 189 bp EcoRI/SspI fragment was confirmed by sequence analysis at BCCM/GeneCorner. Name mentioned in Müller et al. (1986) is pSVd2-hTNF (PstI)-1. Other names of the plasmid are pSVd2-hTNF and pSV23SHuTNFwt. |
| EMBL Accession number: | LT727630.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 30/08/1990 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI, FspI, HindIII, NcoI and SalI. Referring to the expected fragment sizes deduced from the compiled nucleotide sequence, the presence, the length and the orientation of the hTNF insert can be confirmed, but the vector part of the plasmid seems to be larger. The plasmid seems to be about 6000 bp. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr E. Decoster(1) (2) and Prof. Dr W. Fiers(1) (2). It was constructed by X. Van Ostade(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Muller et al., FEBS Lett. 197 (1986), 99-104 [PMID: 3512310] |
| Related plasmid reference: | Decoster et al., J. Cell Biol. 143 (1998), 2057-2065 [PMID: 9864375] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV23SHTNF (LMBP 2173) is available at BCCM/GeneCorner. This plasmid was deposited by Dr E. Decoster and Prof. Dr W. Fiers and was published in Muller et al., 1986. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.