Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV23SmTNFd68-61 (LMBP 3413)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p3413.gb
(View with Genome Compiler) p3413.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse tumor necrosis factor cDNA (Tnf, TNF); presequence with deletion |
| Promoter: | Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV23SMTNF; pMcmTNFΔ-68-61 |
| Further information: | This plasmid was constructed by inserting the SmaI-SstI (SacI) fragment of pMcmTNFΔ-68-61 (containing mTNF with deletion) into the StuI-SstI (SacI) sites of pSV23SMTNF. pSV23SmTNFd68-61 lacks eight amino acids of the intracellular domain of the presequence of mTNF. A silent mutation of codon -73 was performed in order to create a unique NruI restriction site for control. The plasmid contains the SV40 small t-antigen splicing signal. Restriction enzyme digestions revealed that approx. 60 nucleotides are missing from the 3' untranslated region of the mTNF gene, including the 3'-terminal (supposedly restored) EcoRI site. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727486.1. The nucleotide sequence of the wild type mouse TNF corresponds with the EMBL Nucleotide Sequence Database accession number M11731.1. Other name of the plasmid is pSV23SmTNFΔ-68-61. |
| EMBL Accession number: | M11731.1, view at EMBL, GenBank, DDBJ LT727486.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 06/02/1996 |
| Sequence detail: | mTNF presequence (wild type):
-73 -69 -68 -61 -60
5' ...ATC.CGC.GAC.GTG.GAA.GTG.GCA.GAA.GAG.GCA.CTC.CCC.CAA.AAG.ATG... 3'
-------------------------------
mTNFd68-61:
-73 -69 -60
5' ...ATT.CGC.GAC.GTG.GAA.AAG.ATG... 3'
NruI
--- : deleted fragment |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI, HaeII/StuI, RsaI/XhoI, SacI/SmaI, SalI and TaqI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr E. Decoster(1)(2) and Prof. Dr W. Fiers(1)(2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV23SmTNFd68-61 (LMBP 3413) is available at BCCM/GeneCorner. This plasmid was deposited by Dr E. Decoster and Prof. Dr W. Fiers. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.