Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV25ShTNFR75m1 (LMBP 4330)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4330.gb
(View with Genome Compiler) p4330.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human tumor necrosis factor receptor p75 cDNA (TNFR75, TNFR2, TNFRSF1B); mutant TNF-R75 M1 |
| Promoter: | Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV25S-HTNFR75 |
| Further information: | The plasmid was constructed as follows: the unique BstXI restriction site of pSV25S-HTNFR75 was cut, treated with T4 polymerase and a XbaI linker was inserted. Sequencing results revealed that two linkers were inserted and that an extra C residue of the BstXI site was removed, resulting in the creation of an in frame termination codon at 5 codons downstream of the insert. The plasmid contains the SV40 small t-antigen splicing signal. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727185.1. The nucleotide sequence of the hTNFR75 cDNA corresponds with the EMBL Nucleotide Sequence Database (Release 37; Accession number S63368) completed with the 5' UTR, the signal sequence and the initial 15 codons of the mature hTNFR75 gene obtained from the EMBL Nucleotide Sequence Database (Release 42; Accession number M32315). However, the hTNFR75 coding sequence contains one silent mutation (W. Lesslauer: personal communication). |
| EMBL Accession number: | M32315, view at EMBL, GenBank, DDBJ LT727185.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 23/02/2001 |
| Sequence detail: | Nucleotide sequence of the human TNF-R75 M1
hTNFR75 ->|
310 313|
5'... GAG.AGC.TCG.GCC.ACC.TCT.AGA.GGC.CTC.TAG.AGGTTGG ...3'
Glu Ser Ser Ala Thr Ser Arg Gly Leu *
XbaI XbaI
* : termination codon |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglII/HindIII, EcoRI, HindIII/KpnI and XbaI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr W. Declercq(1) (2) and Prof. Dr P. Vandenabeele(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Declercq et al., J. Immunol. 161 (1998), 390-399 [PMID: 9647248] |
| Related plasmid reference: | Vandenabeele et al., J. Immunol. 154 (1995), 2904-2913 [PMID: 7876558] Dembic et al., Cytokine 2 (1990), 231-237 [PMID: 1966549] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV25ShTNFR75m1 (LMBP 4330) is available at BCCM/GeneCorner. This plasmid was deposited by Dr W. Declercq and Prof. Dr P. Vandenabeele and was published in Declercq et al., 1998. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.