Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV51E6Hy3f21 (LMBP 2145)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p2145.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse anti-hPLAP monoclonal antibody E6(Hy3,κ) half genomic gene; truncated mouse::human chimeric heavy chain of the F(ab')2 fragment E6(Hy3,κ)F2 (E6Hy3f2) |
| Promoter: | Simian virus 40 early promoter (SV40 early) Simian virus 40 late promoter (SV40 late) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV51; pPLcT73; pSV25E6Hy3f2 |
| Further information: | The plasmid was constructed as follows: 1) The E6Hy3f2 gene was first cloned as a HindIII fragment from pSV25E6Hy3f2 into the unique HindIII site of pPLcT73 (clockwise orientated), resulting in the intermediate construction pPLcT73E6Hy3f2; 2) The E6Hy3f2 gene was then cloned (anticlockwise) as an XbaI fragment from pPLcT73E6Hy3f2 This plasmid is similar to pSV51cE6Hy3f2, exept for the cloned gene: the mouse part consists of cDNA; the human part consists of genomic DNA. into the XbaI opened pSV51 vector (base position 3087 and 3636). Other name of the plasmid is pSV51E6Hy3f2. |
| EMBL Accession number: | - |
| Latest sequence update: | 25/07/1990 |
| Sequence detail: | Terminal sequence of the truncated CH2 domain:
CH2
--------------
Arg Thr Pro Asp Leu Val Asn *
5' CGG ACC CCT GAC TTA GTT AAC TAA GCTTGG 3'
^ HpaI HindIII
-------------------
linker
^: 27th amino acid of CH2.
*: Termination codon. |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by K. De Sutter(1) and Prof. Dr W. Fiers(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | De Sutter et al., Gene 113 (1992), 223-230 [PMID: 1572543] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12xB HB101 |
| Host reference: | Boyer and Roulland-Dussoix, J. Mol. Biol. 41 (1969), 459-472 [PMID: 4896022] |
| Related host reference: | Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Sambrook et al. (eds), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY (1989) [ISSN/ISBN: 0879693096] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV51E6Hy3f21 (LMBP 2145) is available at BCCM/GeneCorner. This plasmid was deposited by K. De Sutter and Prof. Dr W. Fiers and was published in De Sutter et al., 1992. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.