Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV51cE6Hy3f2 (LMBP 2211)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p2211.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse anti-hPLAP monoclonal antibody E6(Hy3,κ) cDNA; truncated mouse::human chimeric heavy chain of the F(ab')2 fragment E6(Hy3,κ)F2 (E6Hy3f2) |
| Promoter: | Simian virus 40 early promoter (SV40 early) Simian virus 40 late promoter (SV40 late) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV51cE6Hy3 |
| Further information: | The plasmid was constructed as follows: 1) The SalI-MstII fragment of pSV51cE6Hy3 was removed; 2) After filling in the SalI and MstII sticky ends with Klenow DNA polymerase, at least 2 multi-stop-linkers 'CTTAGTTAACTAAG' were added, repairing the SalI site; the succession of the two linkers resulted in the fomation of a second HindIII site. The exact number of the multi-stop-linkers is unknown: certainly more than one, because an additional HindIII site was found. After translation, E6Hy3f2 contains the first 27 amino acids of the original CH2 domain followed by 4 amino acids derived from the cloning strategy and the multi-stop-linker. The chimeric CH1 domain contains 60 amino acids of mouse (E6H) and 37 amino acids of human (hIG3) origin. The nucleotide sequence of the pOMMB part (located between nucleotide 3394 and 3126; taken from 'GENBANK Release 55; Locus : HUMIGHAF') differs in 1 nucleotide from our own determined E6Hy3 nucleotide sequence, but there are no differences in amino acid sequence. |
| EMBL Accession number: | J00231, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 22/01/1997 |
| Sequence detail: | Nucleotide sequence at the end of the coding information:
CH2
------- @
5' ACC CCT GAC TTA GTT AAC TAA G CTTAGTTAACTAAG TCGACTCTAGA 3'
* HpaI HindIII HpaI SalI
------------------- --------------
multi-stop-linker multi-stop-linker
*: The ACC codon (Thr) is the 26th codon of the CH2 domain.
@: Termination codon. |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr K. De Sutter(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | De Sutter et al., Gene 113 (1992), 223-230 [PMID: 1572543] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV51cE6Hy3f2 (LMBP 2211) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. De Sutter and was published in De Sutter et al., 1992. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.