Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV53cTNF (LMBP 2515)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p2515.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Canine tumor necrosis factor genomic DNA (cTNF) |
Promoter: | Simian virus 40 late promoter (SV40 late) Simian virus 40 early promoter (SV40 early) Canine tumor necrosis factor promoter (cTNF) |
Ribosome binding site: |
- |
Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
Parental clone: | pSV53; pGEM1gcTNF |
Further information: | The plasmid was constructed by inserting the BamHI fragment from pGEMgcTNF (containing the genomic cTNF sequence) into the unique BamHI site of pSV53. The orientation is such that the gene is under control of the late SV40 promoter. Only the coding sequence of canine TNF is known. As the sequence of the introns is unknown, there is uncertainty as to the cutting frequency of the restriction enzymes indicated. Other name of the plasmid is pSV53-CTNFA. |
EMBL Accession number: | - |
Latest sequence update: | 02/10/1995 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr W. Fiers(1) (2). It was constructed by K. Devos(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV53cTNF (LMBP 2515) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr W. Fiers . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.