Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV54 (LMBP 1832)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
psv54.gb
(View with Genome Compiler) psv54.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | Simian virus 40 late promoter (SV40 late) Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli; preferably recombination-deficient strains Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV529 |
| Further information: | The plasmid was constructed by cutting pSV529 with EcoRI; after a Bal 31 treatment nucleotide position 8520 (4271 of pBR322) and nucleotide position 105 (268 of SV40) were ligated with T4 ligase. The Bal 31 deletion extends beyond the SV40 PvuII site. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727593.1. Other name of the plasmid is pSV531. |
| EMBL Accession number: | LT727593.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 28/11/1989 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI and PstI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by G. Maertens(1) and Prof. Dr W. Min Jou(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Huylebroeck et al., Gene 66 (1988), 163-181 [PMID: 2844629] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli |
| Host reference: | - |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV54 (LMBP 1832) is available at BCCM/GeneCorner. This plasmid was deposited by G. Maertens and Prof. Dr W. Min Jou. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.