Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV71BlahCH3.1 (LMBP 4084)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4084.gb
(View with Genome Compiler) p4084.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Ampicillin resistance gene (amp) Human immunoglobulin γ1 cDNA (hIG1); CH3 domain of the heavy chain |
| Promoter: | Simian virus 40 late promoter (SV40 late) Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Chloramphenicol (cam) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli; preferably recombination-deficient strains Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV71BlaL1HI; pBRHIG1 |
| Further information: | pSV71BlahCH3.1 was constructed to screen for interacting CH3 domains (minibodies). The plasmid contains the mutated ampicillin resistance gene in which the termination codon has been replaced by GAG, fused in frame to the CH3 domain of the human immunoglobulin γ1 heavy chain (BlahCH3). The complete fusion gene is placed under control of the SV40 late promoter. The ampicillin resistance gene lacks its own promoter. Nevertheless, the transformants grow on ampicillin selective medium. β-lactamase is also enzymatically active after expression in COS-1 cells. pSV71BlahCH3.1 differs from pSV71BlahCH3 in the CH3 domain originating from the human immunoglobulin γ1 and γ3 respectively. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727303.1. |
| EMBL Accession number: | LT727303.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 14/12/2000 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, BanII, EcoRI/PstI, EcoRV, HaeII and NcoI/XbaI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr R. Schoonjans(1) (2) and Prof. Dr J. Grooten(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV71BlahCH3.1 (LMBP 4084) is available at BCCM/GeneCorner. This plasmid was deposited by Dr R. Schoonjans and Prof. Dr J. Grooten . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.