Last data update: 24 January 2024 16:39 CET
Plasmid name: pSal43A (LMBP 2360)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p2360.gb
(View with Genome Compiler) p2360.txt p2360.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Aspergillus nidulans ornithine carbamoyl transferase gene (argB) |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | Aspergillus nidulans ornithine carbamoyl transferase terminator (argB) |
| Selection marker: | Ampicillin (amp) Ornithine carbamoyl transferase (argB; auxotrophic) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Fungi; integrative |
| Parental clone: | pBR327 |
| Further information: | The plasmid was constructed by cloning a 2.6 kb SalI fragment of A. nidulans DNA containing the argB gene, into the unique SalI site of pBR327. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727506.1 The nucleotide sequence of the A. nidulans insert corresponds with the EMBL Nucleotide Sequence Database accession number BN001303 .1. The nucleotide sequence of the pBR327 vector is based on the EMBL Nucleotide Sequence Database accession number V00083.1, taking into account the revisions of pBR322. |
| EMBL Accession number: | BN001303.1, view at EMBL, GenBank, DDBJ V00083.1, view at EMBL, GenBank, DDBJ LT727506.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 15/04/2013 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglI/EcoRV, EcoRI, EcoRI/PstI, HindIII, HindIII/XmnI, SalI and XmnI. The EcoRI site at position 5872 and the PstI site at position 5124 could not be experimentally confirmed. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr J. Peberdy(1). (1) Department of Botany, University of Nottingham, Nottingham, United Kingdom |
| Plasmid reference: | John et al., Enzyme Microb. Technol. 6 (1984), 386-389 |
| Related plasmid reference: | Upshall et al., Mol. Gen. Genet. 204 (1986), 349-354 [PMID: 3020372] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSal43A (LMBP 2360) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr J. Peberdy and was published in John et al., 1984. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.