Last data update: 24 January 2024 16:39 CET
Plasmid name: pSems-leader-mXFP-mLEP-R_(22-860) (LMBP 13679)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
Sanger: .fasta |
| Cloned DNA: |
Igk leader sequence; N-terminal Influenza HA epitope encoding the haemagglutinin tagging peptide; N-terminal Aequorea victoria green fluorescent protein DNA (GFP); monomeric enhanced red-shifted variant (mEGFP), non-fluorescent mutant (mXFP), N-terminal Mouse leptin receptor cDNA (Lepr, Leprb, Modb1, Obr, GeneID 16847, UniProt P48356); fragment |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Phage T7 gene 10 promoter (T7g10) Escherichia coli lac operon promoter |
| Ribosome binding site: |
Internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV) polyprotein gene |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Neomycin (neo; G418; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Copy number: | High copy number |
| Host range: | Escherichia coli Mammalian cells |
| Further information: | XFP (non-fluorescent version of GFP) is N-terminally fused to the mouse LEP-R receptor fragment (Uniprot P48356, residues 22-860). Monomeric enhanced green fluorescent protein (mEGFP) was rendered non-fluorescent by the mutation Y66F (mXFP). The Igk leader is a short signal peptide that prompts the translocation of a protein to the cellular membrane. |
| EMBL Accession number: | - |
| Latest sequence update: | 13/04/2023 |
| Authenticity test: | The plasmid has been fully sequenced but the NGS data still need to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr Jacob Piehler(1) and Christoph Pollmann(1). (1) Biophysics Division, Department of Biology, Osnabrück University, Germany |
| Plasmid reference: | - |
| Related plasmid reference: | Tsirigotaki et al., Nat. Struct. Mol. Biol. 30 (2023), 551-563 [PMID: 36959263] [DOI: 10.1038/s41594-023-00941-9] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSems-leader-mXFP-mLEP-R_(22-860) (LMBP 13679) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr Jacob Piehler and Christoph Pollmann. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.