Last data update: 24 January 2024 16:39 CET
Plasmid name: pTM6HIFNG (LMBP 2008)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p2008.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human interferon γ cDNA (IFNG); mutated mature sequence |
| Promoter: | Phage λ major leftward promoter (λ PL) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli tryptophan operon attenuator peptide (trp) |
| Terminator: | Phage fd terminator |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli; use strains with a cI function |
| Parental clone: | pPLc321HIFNGHS; pTM2HIFNG |
| Further information: | pTM6HIFNG is a Target Mutagenesis plasmid. The human interferon γ gene (IFNG) is a mutated variant, derived from the ancestral pPLc321HIFNGHS vector; it contains an extra HhaI site by silent mutations of codons 130 and 131, and a unique SalI site at codons 138-140. No codon changes are involved. The length of the 3UTR fragment of human IFGN and its fusion to the XbaI site of pTM2HIFNG is uncertain. This plasmid can be used in conjunction with pTM7HIFNG to obtain selection for the opposite strand. The nucleotide sequence of the wt human IFNG DNA corresponds with Genbank accession number NM_000619.2. Other name of the plasmid is pTM6G. |
| EMBL Accession number: | NM_000619.2, view at GenBank |
| Latest sequence update: | 19/11/2010 |
| Sequence detail: | Start of the mature human IFN gamma cDNA:
|-> mature human IFNG
| 1 5
5' AAAAAGGGTATCGATTCC.ATG.CAG.GAC.CCA.TAT.GTA.AAA 3'
ClaI Met Gln Asp Pro Tyr Val Lys
Nucleotide sequence at the end of the human IFNG wt DNA:
130 139 140 143
5' CGA.AAA.AGG.AGT.CAG.ATG.CTG.TTT.CGA.GGT.CGA.AGA.GCA.TCC.CAG.TAA.TGGTTGTC... 3'
Arg Lys Arg Ser Gln Met Leu Phe Arg Gly Arg Arg Ala Ser Gln
Nucleotide sequence at the end of the human IFNG variant:
130 131 139 140 143
5' CGA.AAG.CGC.AGT.CAG.ATG.CTG.TTT.CGA.GGT.CGA.CGA.GCA.TCC.CAG.TAA.TGGTTGTC... 3'
Arg Lys Arg Ser Gln Met Leu Phe Arg Gly Arg Arg Ala Ser Gln
* * * SalI *
HhaI
*: mutated nucleotide |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061(λ) |
| Host reference: | Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329] |
| Related host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pTM6HIFNG (LMBP 2008) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.