Last data update: 24 January 2024 16:39 CET
Plasmid name: pUC19-BamHI-polyCisRibo-EcoRI (LMBP 9161)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p9161.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human ribosomal protein S27a cDNA (RPS27A, UBCEP80; Uba80; S27A) Human ubiquitin A-52 residue ribosomal protein fusion product 1 cDNA (RPL40, CEP52, HUBCEP52, MGC57125, MGC126879, MGC126881, L40) Porcine teschovirus-1 2A peptide (P2A) Influenza HA epitope encoding the haemagglutinin tagging peptide; N-terminal Vesicular stomatitis virus glycoprotein (VSV-G) epitope E-tag; C-terminal |
| Promoter: | Escherichia coli lac operon promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pUC19 |
| Further information: | The plasmid was constructed by cloning the synthetic HA-L40-P2A-VSV-27a-P2A-Etag cassette into the pUC19 vector. The polycistronic construct [BamHI]-HA-L40-P2A-VSV-27a-P2A-Etag-[EcoRI] (PolyCisRibo) is meant to be used for ubiquitin-replacement constructs, where mutated/tagged ubiquitins are cloned upstream of the PolyCisRibo and a selectable marker downstream of the PolyCisRibo. HA, VSV and E-tags are used to monitor processing and maturation of the construct. P2A is a self-cleaving peptide, fulfilling the same role as an IRES, but has a much shorter sequence. |
| EMBL Accession number: | - |
| Latest sequence update: | 12/04/2017 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by DR J. Staal(1)(2) and Prof. Dr R. Beyaert(1)(2). (1) VIB Center for Inflammation Research, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Xu et al., Mol. Cell 36 (2009), 302-314 [PMID: 19854138] [DOI: 10.1016/j.molcel.2009.10.002] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pUC19-BamHI-polyCisRibo-EcoRI (LMBP 9161) is available at BCCM/GeneCorner. The plasmid was deposited by DR J. Staal and Prof. Dr R. Beyaert. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.