Last data update: 24 January 2024 16:39 CET
Plasmid name: pUC19-KpnI-ClRT7-M1-PmeI (LMBP 9160)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p9160.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Porcine teschovirus-1 2A peptide (P2A) Influenza HA epitope encoding the haemagglutinin tagging peptide; N-terminal Vesicular stomatitis virus glycoprotein (VSV-G) epitope E-tag; N-terminal SV40 large T-antigen nuclear localization signal (NLS); N-terminal Mouse lymphocyte protein tyrosine kinase cDNA (Lck); myristoylation-targeting sequence, N-terminal |
| Promoter: | Escherichia coli lac operon promoter Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) Internal ribosome entry site (IRES) of the human POU class 5 homeobox 1 gene (POU5F1) |
| Terminator: | Phage T7 gene 10 terminator (T7g10) Bovine growth hormone polyadenylation signal (BGH polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pUC19 |
| Further information: | The plasmid was constructed by cloning the synthetic KpnI-CIRT7-M1-PmeI cassette into the pUC19 vector. The KpnI-CIRT7-M1-PmeI cassette consists of the following elements: - a Myr epitope tag - an HA epitope tag - a nuclear export signal - an LVSRGRSLVSRG linker - a VSV epitope tag - a nuclear localisation signal - a pT7pol cloning site (BamHI, EcoRI) - a P2A autoprocessing peptide - a selectable marker cloning site (EcoRV) - a BGH terminator - a T7 promoter - a POU5F1 (= OCT4B) minimal IRES - an E-tag - a reporter cloning site (NotI, XbaI) - a T7 terminator The CIRT7 cassette is meant to be recloned into a mammalian expression vector for various types of MALT1 cleavage readouts. It is intended primarily to amplify a specific signal in order to observe endogenous activity. P2A is a self-cleaving peptide, fulfilling the same role as an IRES, but has a much shorter sequence. |
| EMBL Accession number: | - |
| Latest sequence update: | 12/04/2017 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by DR J. Staal(1)(2) and Prof. Dr R. Beyaert(1)(2). (1) VIB Center for Inflammation Research, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pUC19-KpnI-ClRT7-M1-PmeI (LMBP 9160) is available at BCCM/GeneCorner. The plasmid was deposited by DR J. Staal and Prof. Dr R. Beyaert. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.