Last data update: 24 January 2024 16:39 CET
Plasmid name: pUC19-hDRD3 (LMBP 11209)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human dopamine receptor D3 cDNA (DRD3, GeneID 1814) |
| Promoter: | Escherichia coli lac operon promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pUC19 |
| Further information: | The plasmid was constructed by cloning the human DRD3 coding sequence into the pUC19 vector. The nucleotide sequence of the parental pUC19 plasmid corresponds with the EMBL Nucleotide Sequence Database accession number M77789.1, starting at another nucleotide position and complemented. Other name of the plasmid is pUC19humD3. |
| EMBL Accession number: | M77789.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 02/09/2019 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr K. Van Craenenbroeck(1). (1) Laboratory for GPCR Expression and Signal Transduction, Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Norrander et al., Gene 26 (1983), 101-106 [PMID: 6323249] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pUC19-hDRD3 (LMBP 11209) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr K. Van Craenenbroeck. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.