Last data update: 24 January 2024 16:39 CET
Plasmid name: pUC19Trgls2a(5'-Inv+Rep-3') (LMBP 5874)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p5874.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Trichoderma reesei glucosidase ll cDNA (Trgls2); α subunit (Trgls2a); mutant |
Promoter: | Escherichia coli lac operon promoter |
Ribosome binding site: |
- |
Terminator: | - |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli |
Further information: | The 5' flanking sequences of the glucosidase II alpha subunit ORF were isolated from a 5' inverse PCR fragment: the relevant sequence was isolated as an EcoRI/DraIII fragment and ligated into the EcoRI/DraIII opened vector pUC19Trgls2a(Inv+Rep-3'), resulting into plasmid pUC19Trgls2a(5'-Inv+Rep-3'). |
EMBL Accession number: | - |
Latest sequence update: | 17/03/2009 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr N. Callewaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061 |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pUC19Trgls2a(5'-Inv+Rep-3') (LMBP 5874) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr N. Callewaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.