Last data update: 24 January 2024 16:39 CET
Plasmid name: pUC57-FvCASP8-2A-FRBCFLAR (LMBP 9087)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p9087.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human caspase 8 cDNA (CASP8, Casp-8, FLICE, MACH, MCH5, GeneID 841); modified sequence Human CASP8 and FADD like apoptosis regulator cDNA (CFLAR, CASP8AP1, c-FLIP, CASH, Casper, CLARP, FLAME, FLIP, I-FLICE, MRIT); modified sequence Porcine teschovirus-1 2A peptide (P2A) |
| Promoter: | Escherichia coli lac operon promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pUC57 |
| Further information: | The plasmid was constructed by cloning a synthetic construct of human CASP8 and human CFLAR, linked by a 2A autoprocessing peptide, into the pUC57 shuttle vector. The DED domains of the human CASP8 coding sequence were replaced by an artificial dimerization domains (Fv or FKBP). The DED domains of the human CFLAR coding sequence were replaced with an FRB artificial dimerization domain. Other name of the plasmid is FvCasp82AFRBFLIP pUC57. |
| EMBL Accession number: | - |
| Latest sequence update: | 05/04/2017 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Prof. Dr R. Beyaert(1)(2). It was constructed by Dr. B. Van Raam(3) (1) Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium (3) BioMed X Innovation Center, Heidelberg, Germany |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pUC57-FvCASP8-2A-FRBCFLAR (LMBP 9087) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr R. Beyaert. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.