Last data update: 24 January 2024 16:39 CET
Plasmid name: pUC57-GLAmutARARSL-MycHis (LMBP 11120)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p11120.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human galactosidase alpha cDNA (GLA, GALA, GeneID 2717); mutated synthetic sequence Histidine tag (His-tag); C-terminal Myc epitope; C-terminal |
| Promoter: | Escherichia coli lac operon promoter (lac) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pUC57 |
| Further information: | The plasmid was constructed by cloning the mutated synthetic sequence of human GLA into the pUC57 vector. The human GLA coding sequence contains the A348R, A368R and S405L mutations. The nucleotide sequence of the wild type human GLA coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number X16889.1. Other names of the plasmid are pUC57_synthetischGLAmutARARSL and pUC57_synthetic_GLA_mut_ARARSL. |
| EMBL Accession number: | X16889.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 10/09/2018 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr N. Callewaert(1) (2). (1) VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium (2) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium |
| Plasmid reference: | PhD thesis Charlotte De Visscher (2015) |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pUC57-GLAmutARARSL-MycHis (LMBP 11120) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr N. Callewaert and was published in Charlotte De Visscher, 2015. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.