Last data update: 24 January 2024 16:39 CET
Plasmid name: pUCIVHAsm (LMBP 3923)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p3923.gb
(View with Genome Compiler) p3923.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Influenza A virus (A/Victoria/3/1975(H3N2)) haemagglutinin cDNA (HA, IVHA); mutated sequence, N-terminal anchor free |
| Promoter: | Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pUC18; pSV23IVHAsm |
| Further information: | The plasmid was constructed by inserting the SalI-BamHI fragment of pSV23IVHAsm, containing the A/Victoria/3/75 influenza haemagglutinin cDNA encoding a mutated, secreted protein (IVHAsm), into the SalI-BamHI opened pUC18 vector. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727288.1. |
| EMBL Accession number: | LT727288.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 08/03/1999 |
| Sequence detail: | Nucleotide sequence at the start of the mature haemagglutinin (HA):
signal HA sequence ->|-> mature HA sequence
** |
5' … TTC.TGT.CTG.GTT.TGG.GCC|CAA.GAC.CTT.CCA.GGA.AAT.GAC … 3'
Phe Cys Leu Val Trp Ala|Gln Asp Leu Pro Gly Asn Asp
ApaI
*: Mutated nucleotide.
Nucleotide sequence at the end of the mature haemagglutinin (HA):
mature HA sequence ->|-> pUC18
|
5' … TAC.AAA.GAC.TGG.ATC|CCC.GGG.TAC.CGA.GCT … 3'
Tyr Lys Asp Trp Ile|Pro Gly Tyr Arg Ala
BamHI |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: PstI and SpeI/XmnI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr X. Saelens(1) (2) and Prof. Dr W. Min Jou(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Saelens et al., Eur. J. Biochem. 260 (1999), 166-175 [PMID: 10091596] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pUCIVHAsm (LMBP 3923) is available at BCCM/GeneCorner. This plasmid was deposited by Dr X. Saelens and Prof. Dr W. Min Jou and was published in Saelens et al., 1999. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.