Last data update: 24 January 2024 16:39 CET
Plasmid name: pUD208 (LMBP 2504)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | pud208.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | Saccharomyces cerevisiae iso-1-cytochrome C promoter (CYC1) |
| Ribosome binding site: |
- |
| Terminator: | Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1) |
| Selection marker: | Ampicillin (amp) Saccharomyces cerevisiae URA3; auxotrophic Methotrexate (MTX) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Saccharomyces cerevisiae; ura3(-), integrative |
| Parental clone: | pUDHIL208 |
| Further information: | The plasmid was constructed as follows : 1) pUDHIL208 was cleaved with SalI and filled-in with Klenow DNA polymerase; 2) BamHI linkers ('CGGGATCCCG') were ligated; 3) This construction was then cut with BamHI to remove the BamHI fragment, containing the hIL2 gene. This plasmid contains a dominant selection marker for integrative transformation (e.g. in TY1 after cleavage with ClaI or XhoI) Since the nucleotide sequence from position 5278 to 5637 is not known, there is uncertainty as to the cutting frequency of the restriction enzymes indicated. |
| EMBL Accession number: | - |
| Latest sequence update: | 17/12/1990 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Contreras(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pUD208 (LMBP 2504) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Contreras. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.