Last data update: 24 January 2024 16:39 CET
Plasmid name: pUT18-ProP (LMBP 12379)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Escherichia coli osmolyte:H+ symporter ProP cDNA (proP, GeneID 948626) Escherichia coli adenylate cyclase cDNA (cyaA, GeneID 947755); fragment |
| Promoter: | Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pUT18 |
| Further information: | The plasmid was constructed by cloning the E. coli ProP coding sequence into the pUT18 vector. pUT18 is a derivative of the high copy-number plasmid pUC19, and encodes the T18 fragment (corresponding to amino acids 225-399 of E. coli CyaA) that is expressed under the transcriptional control of a lac promoter. Other name of the plasmid is pT18ProP. |
| EMBL Accession number: | - |
| Latest sequence update: | 04/03/2021 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr D.E. Culham(1) and Prof. Em. Dr J.M. Wood(1). (1) Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada |
| Plasmid reference: | Romantsov et al., Mol. Microbiol. 107 (2018), 623-638 [PMID: 29280215] [DOI: 10.1111/mmi.13904] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 WG350 (LMBP 12428) |
| Host reference: | Culham et al., J. Mol. Biol. 229 (1993), 268-276 [PMID: 8421314] [DOI: 10.1006/jmbi.1993.1030] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pUT18-ProP (LMBP 12379) is available at BCCM/GeneCorner. This plasmid was deposited by Dr D.E. Culham and Prof. Em. Dr J.M. Wood and was published in Romantsov et al., 2018. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.