Last data update: 24 January 2024 16:39 CET
Plasmid name: pUZ8::Tn9 (LMBP 8032)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Chloramphenicol (cam) Neomycin (neo; kanamycin (kan)) Tetracycline (tet) Methyl-β-D-galactopyranoside (Tra+) |
| Replicon: | - |
| Host range: | Escherichia coli |
| Parental clone: | - |
| Further information: | The plasmid belongs to the IncP1 incompatibility family. This plasmid is a broad host range cloning vector. |
| EMBL Accession number: | - |
| Latest sequence update: | 18/03/2013 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by M. Mergeay(1) and A. Provoost(1). (1) SCK.CEN, Mol, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 C600[pGV1106] |
| Host reference: | - |
| Related host reference: | Appleyard, Genetics 39 (1954), 440-452 [PMID: 17247495] |
| Helper plasmid: | pGV1106 |
| Cultivation medium: | LB-Lennox + chloramphenicol (34 μg/ml) + kanamycin (50 μg/ml) + streptomycin (25 μg/ml)* |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | *: selection of transformants on chloramphenicol and streptomycin; subsequent cultivation of a single colony in liquid medium with kanamycin and streptomycin. Chloramphenicol resistance and streptomycin resistance are located on different plasmids. Use both antibiotics to select for both plasmids. |
| Other culture collection numbers: | SCK CM0277 |
Refer in your Materials and Methods: |
pUZ8::Tn9 (LMBP 8032) is available at BCCM/GeneCorner. This plasmid was deposited by M. Mergeay and A. Provoost. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.