Last data update: 24 January 2024 16:39 CET
Plasmid name: pVIK107 (LMBP 4114)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4114.gb
(View with Genome Compiler) p4114.txt p4114.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Escherichia coli lac Z gene (lacZ); starting at the 9th codon Escherichia coli lac Y gene (lacY) Escherichia coli lac A gene (lacA); fragment |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Neomycin (neo; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid R6K origin; defective pir gene Broad-host-range plasmid RK2/RP4 origin of transfer (oriT) |
| Host range: | Escherichia coli; use strains supplying the R6K pir function Gram-negative bacterial strains |
| Parental clone: | pGP704; pLKC480 |
| Further information: | This plasmid was constructed as follows: pGP704 was digested with PstI (blunted with T4 DNA polymerase). The resulting 3 kb fragment containing the vegetative replication origin of plasmid R6K, lacking its pir gene, and the origin of conjugal transfer of plasmid RP4, was ligated with a 6.3 kb SmaI fragment of pLKC480, containing a promoterless lacZY cassette and a neomycin resistance gene. pVIK107 is a suicide vector designed to create translational fusions between chromosal or plasmid-encoded genes and the lacZ reporter gene. This plasmid can be used to measure transcriptional regulation of particular promoters and to study gene regulation at the translational level. To this end, the plasmid requires the introduction of a promoter and a translation initiation region. Introduce the plasmid by electroporation, or through conjugation, using plasmid RK2/RP4 transfer functions (provided in trans by RK2/RP4, RK2/RP4-derived vectors, other replicons carrying RK2/RP4 transfer functions (e.g. pRK2073) or E. coli strains expressing the tra genes of RK2/RP4 (e.g. S17-1(λpir)). The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727307.1. |
| EMBL Accession number: | LT727307.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 22/04/2008 |
| Sequence detail: | Nucleotide sequence of the multicloning site:
5' ... GAA.TTC.CCG.GGA.GAG.CTC.GAT.ATC.GCA.TGC.GGT.ACC.TCT.AGA.AGA.AGC.TTG.GGA
EcoRI SmaI SacI EcoRV SphI KpnI XbaI HindIII BamHI
pGP704-->|--> pLKC480
TCC.GTC.GAC.C|GG.GGA.TCC.GTC.GAC.CTG.CAG.CCA.AGC.TTC ... 3'
SalI BamHI SalI PstI HindIII
Punctuation indicates reading frame. |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRV, SacI and XbaI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr S.C. Winans(1). It was constructed by Dr V.S. Kalogeraki(1). (1) Section of Microbiology, Cornell University, Ithaca, USA |
| Plasmid reference: | Kalogeraki et al., Gene 188 (1997), 69-75 [PMID: 9099861] |
| Related plasmid reference: | Tiedeman et al., Nucleic Acids Res. 16 (1988), 3587 [PMID: 3131742] Minton, Gene 31 (1984), 269-273 [PMID: 6098531] Miller et al., J. Bacteriol. 170 (1988), 2575-2583 [PMID: 2836362] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 SY327(λpir) |
| Host reference: | Miller et al., J. Bacteriol. 170 (1988), 2575-2583 [PMID: 2836362] |
| Related host reference: | Kalogeraki et al., Gene 188 (1997), 69-75 [PMID: 9099861] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pVIK107 (LMBP 4114) is available at BCCM/GeneCorner. This plasmid was deposited by Dr S.C. Winans and was published in Kalogeraki et al., 1997. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.