Last data update: 24 January 2024 16:39 CET
Plasmid name: pX330-Mir511-sgRNA (LMBP 9409)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Single-guide RNA targeting mouse microRNA 511 (Mir511, Mirn511, mmu-mir-511) Streptococcus pyogenes CRISPR associated protein 9 cDNA (Cas9) SV40 large T-antigen nuclear localization signal (NLS); N-terminal FLAG epitope tag; N-terminal |
| Promoter: | Human U6 small nuclear RNA gene promoter Chicken β-actin promoter (ACTB) Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only |
| Ribosome binding site: |
- |
| Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Bacteriophage M13 origin Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | pX330 |
| Further information: | The plasmid was constructed by cloning single-guide RNA for mouse MIR511 into the pX330 vector. The plasmid is meant for targeting mouse Mir511 for CRISPR/CAS knockout. Other name of the plasmid is pX330 + Mir511 sgRNA. |
| EMBL Accession number: | - |
| Latest sequence update: | 01/03/2017 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr T. Hochepied(1) (2) and Prof. Dr C. Libert(1) (2). It was constructed by J. D'Hont(1)(2). (1) Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pX330-Mir511-sgRNA (LMBP 9409) is available at BCCM/GeneCorner. This plasmid was deposited by Dr T. Hochepied and Prof. Dr C. Libert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.