Last data update: 24 January 2024 16:39 CET
Plasmid name: pYES2-TruMDR5 (LMBP 12061)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Trichophyton rubrum ABC transporter cDNA (TERG_04952, MDR5, GeneID 10372913) |
| Promoter: | Saccharomyces cerevisiae galactokinase promoter (GAL1) Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1) |
| Selection marker: | Ampicillin (amp) URA3; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Saccharomyces cerevisiae 2 micron plasmid origin (2μ) |
| Host range: | Escherichia coli Saccharomyces cerevisiae; ura3(-) |
| Parental clone: | pYES2 |
| Further information: | The plasmid was constructed by cloning the T. rubrum MDR5 coding sequence into the pYES2 yeast expression vector. The plasmid contains the GAL1 promoter for inducible expression of genes by galactose and repression by glucose. The ATP-binding cassette transporters or ABC transporters consist of multiple subunits, transmembrane proteins and membrane-associated ATPases which utilize the energy of adenosine triphosphate (ATP) binding and hydrolysis to provide the energy needed for the translocation of substrates across membranes, either for uptake or for export of the substrate. The T. rubrum MDR5 coding sequence shows close homology to the A. fumigatus ABCE coding sequence. The plasmid pYES2-TruMDR5 is also available in Saccharomyces cerevisiae under accession number LMBP 12078. The nucleotide sequence of the T. rubrum MDR5 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number MK787256.1. Other name of the plasmid is pYES2_TruMDR5. |
| EMBL Accession number: | MK787256.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 17/01/2020 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Prof. Dr M. Monod(1). (1) Department of Dermatology, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland |
| Plasmid reference: | Monod et al., Antimicrob. Agents Chemother. 63 (2019), e00863-19 [PMID: 31501141] [DOI: 10.1128/AAC.00863-19] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | LMBP 12078 |
Refer in your Materials and Methods: |
pYES2-TruMDR5 (LMBP 12061) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr M. Monod and was published in Monod et al., 2019. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.