Last data update: 24 January 2024 16:39 CET
Plasmid name: pYES2-TruMFS2 (LMBP 12065)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
NGS: b12-gc-may2021.fasta |
| Cloned DNA: |
Trichophyton rubrum MFS multidrug transporter cDNA (TERG_08336, MFS2, GeneID 10377525) |
| Promoter: | Saccharomyces cerevisiae galactokinase promoter (GAL1) Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1) |
| Selection marker: | Ampicillin (amp) URA3; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Saccharomyces cerevisiae 2 micron plasmid origin (2μ) |
| Host range: | Escherichia coli Saccharomyces cerevisiae; ura3(-) |
| Parental clone: | pYES2 |
| Further information: | The plasmid was constructed by cloning the T. rubrum MFS2 coding sequence into the pYES2 yeast expression vector. The plasmid contains the GAL1 promoter for inducible expression of genes by galactose and repression by glucose. The major facilitator superfamily (MFS) is a superfamily of membrane transport proteins that facilitate movement of small solutes across cell membranes in response to chemiosmotic gradients. The T. rubrum MFS2 coding sequence shows close homology to the A. fumigatus MDRA coding sequence. The plasmid pYES2-TruMFS2 is also available in Saccharomyces cerevisiae under accession number LMBP 12082. The nucleotide sequence of the T. rubrum MFS2 coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number MK787259.1. Other name of the plasmid is pYES2_TruMFS2. |
| EMBL Accession number: | MK787259.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 17/01/2020 |
| Authenticity test: | This plasmid has been fully sequenced but the NGS sequence data still need to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Prof. Dr M. Monod(1). (1) Department of Dermatology, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland |
| Plasmid reference: | Monod et al., Antimicrob. Agents Chemother. 63 (2019), e00863-19 [PMID: 31501141] [DOI: 10.1128/AAC.00863-19] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | LMBP 12082 |
Refer in your Materials and Methods: |
pYES2-TruMFS2 (LMBP 12065) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr M. Monod and was published in Monod et al., 2019. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.