Last data update: 24 January 2024 16:39 CET
Plasmid name: pcDNA3-AU1-hMyD88 (LMBP 5620)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p5620.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human myeloid differentiation primary response gene 88 cDNA (MYD88) AU1-tag; N-terminal |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Phage SP6 promoter Phage T7 gene 10 promoter (T7g10) Simian virus 40 early promoter (SV40 early) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Neomycin (neo; G418) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pcDNA3 |
| Further information: | There is uncertainty about the presence of a second enhancer in the SV40 early promoter. The absence of a second enhancer in this promoter may cause bacterial leakage expression of the Tn5 neomycin resistance gene. This vector has been shown to provide resistance to kanamycin, although growth was reduced compared to growth on medium containing ampicillin. The nucleotide sequence of the human MYD88 cDNA was analysed by BCCM/GeneCorner. This sequence differs in one nucleotide from the EMBL Nucleotide Sequence Database accession number U84408.1: the C nucleotide at position 7 of the EMBL MYD88 coding sequence was analysed as a G, resulting in a A3G replacement. Other name of the plasmid is pcDNA3-hMyD88-AU1. |
| EMBL Accession number: | U84408.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 02/04/2010 |
| Sequence detail: | Nucleotide sequence at the 5' end of the human MYD88 cDNA:
|-> human MYD88
|-> AU1-tag ->|
2 3 4 5 6
5' ... CTCGGATCCGCC.ATG.GAC.ACA.TAC.CGC.TAC.ATC.GCT.GGA.GGA.GGT.CCC ... 3'
BamHI D T Y R Y I *
*: nucleotide 'G' according to the LMBP sequence analysis results; 'C' according to the EMBL Nucleotide Sequence Database accession number U84408 (version SV 1). |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, EcoRI/HindIII, HaeII and PstI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr. M. Muzio(1) (1) Division of Experimental Oncology, San Raffaele Scientific Institute, Milan, Italy |
| Plasmid reference: | - |
| Related plasmid reference: | Muzio et al., J. Exp. Med. 187 (1998), 2097-2101 [PMID: 9625770] Muzio et al., Science 278 (1997), 1612-1615 [PMID: 9374458] Lord et al., Oncogene 5 (1990), 1095-1097 [PMID: 2374694] Janssens et al., FEBS Lett. 548 (2003), 103-107 [PMID: 12885415] Janssens et al., Trends Biochem. Sci. 27 (2002), 474-482 [PMID: 12217523] Janssens et al., Curr. Biol. 12 (2002), 467-471 [PMID: 11909531] Bonnert et al., FEBS Lett. 402 (1997), 81-84 [PMID: 9013863] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pcDNA3-AU1-hMyD88 (LMBP 5620) is available at BCCM/GeneCorner. This plasmid was deposited by Dr. M. Muzio Division of Experimental Oncology, San Raffaele Scientific Institute, Milan, Italy. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.