Last data update: 24 January 2024 16:39 CET
Plasmid name: pcDNA3-F-HLH-Ig2-VSV-MALT1C-HA (LMBP 9563)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human MALT1 paracaspase cDNA (MALT1, MLT, PCASP1, paracaspase 1, GeneID 10892); fragment Vesicular stomatitis virus glycoprotein (VSV-G) epitope; N-terminal HLH dimerization domain; N-terminal FLAG epitope tag; N-terminal Influenza HA epitope encoding the haemagglutinin tagging peptide; C-terminal |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Simian virus 40 early promoter (SV40 early) Phage T7 gene 10 promoter (T7g10) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Neomycin (neo; G418) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pcDNA3.1-F-HLH-Ig2-VSV-MALT1C-EARmut-HA; pCD-MK |
| Further information: | The plasmid was constructed by PCR amplifying a C-terminal fragment of the human MALT1 coding sequence from pCD-MK and cloning it into the EcoRV/EcoRI opened pCDNA3-F-HLH-Ig2-VSV-MALT1C-EARmut-HA vector. The C-terminal fragment of human MALT1 is fused to the VSV-G epitope, the immunoglobulin-like domain Ig2 of human MALT1, the HLH dimerization domain and the FLAG epitope at its N-terminus and to the HA epitope at its C-terminus. The plasmid contains the ETV6 helix-loop-helix domain (HLH) which is a dimerization domain. The immunoglobulin-like domain Ig2 of hMALT1 contributes to the oligomerization of the caspase-like domain and helps with the correct dimerization. Other name of the plasmid is pCDNA3-F-HLH-Ig2-VSV-MALT1C-WT-HA. |
| EMBL Accession number: | - |
| Latest sequence update: | 03/09/2019 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2) and Dr J. Staal(1) (2). (1) Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pcDNA3-F-HLH-Ig2-VSV-MALT1C-HA (LMBP 9563) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert and Dr J. Staal . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.